Your HVAC equipment makes conditioned air. Your duct system is responsible for delivering it. When one room underperforms, the question worth answering early is whether the air intended for that room is actually arriving.
Duct leakage is one of the few items on this list that can be measured rather than estimated. A duct leakage test pressurises the duct system and quantifies how much air is escaping, so the duct system either is or is not part of your problem — and you have a number either way.
Testing also distinguishes leakage from the problems sealing cannot fix. A run that is crushed or disconnected shows up differently from a system that is simply leaking at its seams, and the honest answer in that case is repair or modification — not sealing around it.